{"id":401,"date":"2025-04-11T15:55:49","date_gmt":"2025-04-11T22:55:49","guid":{"rendered":"https:\/\/labs.engineering.asu.edu\/yaron\/?page_id=401"},"modified":"2025-04-11T15:55:49","modified_gmt":"2025-04-11T22:55:49","slug":"thp-1-cell-culture","status":"publish","type":"page","link":"https:\/\/labs.engineering.asu.edu\/yaron\/protocols-recipes\/thp-1-cell-culture\/","title":{"rendered":"THP-1 Cell Culture"},"content":{"rendered":"\n\n\n
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Highly primary-like human monocyte\/macrophage cell line.<\/h3>\n\n\n\n

These cells are useful for investigating macrophage-like behavior, including studying inflammatory cytokine synthesis and secretion, assembly of the inflammasome, immune responses to PAMPs and DAMPs, biomaterial-immune cell interactions, etc.<\/p>\n\n<\/div>\t\t<\/div>\n\t\t\t\t\t<\/div>\n<\/section>\n\n\n\n

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ATCC:\u00a0<\/strong>TIB-202<\/p>\n\n\n\n

Link:<\/strong> https:\/\/www.atcc.org\/products\/all\/TIB-202.aspx <\/a><\/p>\n\n\n\n

Organism:\u00a0<\/strong>Human<\/p>\n\n\n\n

Morphology:\u00a0<\/strong>Monocyte (can differentiate to macrophage)<\/p>\n\n\n\n

Culture:<\/strong>\u00a0Suspension (adherent as macrophage)<\/p>\n\n\n\n

BSL: <\/strong>1<\/p>\n\n\n\n

Culture Medium:\u00a0<\/strong>RPMI-1640 with 10% FBS, 1% (2 mM) L-Glutamine, and 1% Pen\/Strep. ATCC-recommended addition of 0.05 mM 2-mercaptoethanol is not considered necessary by all labs.<\/p>\n\n\n\n

Passaging conditions:<\/strong>\u00a0Change\/add medium as necessary to maintain pH, keeping cells between 200,000 and 800,000 cells per mL. Avoid growing to greater than 1 million cell per mL.<\/p>\n\n\n\n

Cryopreservation medium:\u00a0<\/strong>ATCC recommends\u00a0<\/strong>pH-equilibrated culture medium + 5% ultra-pure DMSO (or 90% FBS and 10% ultra-pure DMSO)<\/p>\n\n\n\n

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Suspension cells, do not <\/em><\/strong>trypsinize.<\/em><\/p>\n\n<\/div><\/div><\/div>\n\n

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Highly sensitive and virtually untransfectable with expression plasmids without virus or nucleofection.<\/em><\/strong><\/p>\n\n<\/div><\/div><\/div>\n\n

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If cells appear to have protrusions\/blebs in suspension culture then start new culture from liquid nitrogen. Do not culture for more than 40-60 days from a single culture.<\/em><\/p>\n\n<\/div><\/div><\/div>\n\n

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Cells can be differentiated to adherent macrophages by treating with 1-100 nM PMA for 18-36 hours on the final culture substrate. Do not try to trypsinize\/scrape and transfer cells after differentiation. Wash cells prior to final experimentation.<\/p>\n\n<\/div><\/div><\/div><\/div>\n<\/div>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

ATCC:\u00a0TIB-202 Link: https:\/\/www.atcc.org\/products\/all\/TIB-202.aspx Organism:\u00a0Human Morphology:\u00a0Monocyte (can differentiate to macrophage) Culture:\u00a0Suspension (adherent as macrophage) BSL: 1 Culture Medium:\u00a0RPMI-1640 with 10% FBS, 1% (2 mM) L-Glutamine, and 1% Pen\/Strep. ATCC-recommended addition of 0.05 mM 2-mercaptoethanol is not considered necessary by all labs. Passaging conditions:\u00a0Change\/add medium as necessary to maintain pH, keeping cells between 200,000 and 800,000 cells per…<\/p>\n","protected":false},"author":408,"featured_media":0,"parent":261,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-401","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/pages\/401","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/users\/408"}],"replies":[{"embeddable":true,"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/comments?post=401"}],"version-history":[{"count":0,"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/pages\/401\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/pages\/261"}],"wp:attachment":[{"href":"https:\/\/labs.engineering.asu.edu\/yaron\/wp-json\/wp\/v2\/media?parent=401"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}